Establishing a robust protocol for viral nucleic acid extraction from food products using an internal control

Gaillard, Ebony

2015

2010-2019

Food products can potentially be hosts of food-borne viruses such as single-strand RNA viruses. The contamination of food products potentially cause viruses such as Hepatitis B and E to spread throughout populations. The purpose of this study is to create a robust protocol for the detection of viral RNA in food products. In this study, we studied the presence of viral RNA in canine liver tissue samples spiked with Feline Calicivirus (FCV). The three methods used to break up the liver tissue are as followed: dicing with a scalpel, shredding utilizing the QIAShredder, and mashing with a pestle-like instrument. These methods were evaluated to find the best way to recover viral RNA from the canine liver tissue. By completing downstream applications such as polymerase chain reaction (PCR) and gel electrophoresis, it was found that each method successfully recovered viral RNA. A Nanodrop spectrometer was used to measure the concentration of recovered RNA in each spiked liver sample and it was concluded that mashing via the pestle instrument was the most efficient method to recover the most RNA.

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conference proceedings

Enhancing Global Research and Education in STEM at Spelman College (G-STEM)

Department of Chemistry and Biochemistry

D'Mello, Felicity
Bose, Nripendra

Spelman College

http://hdl.handle.net/20.500.12322/sc.gstem:2015_gaillard_ebony

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